Conformation of ring single-stranded DNA measured by DNA origami structures.

ABSTRACT: Measuring the mechanical properties of single-stranded DNA (ssDNA) is a complex challenge that has been addressed lately by different methods. We measured the persistence length of ring ssDNA using a combination of a special DNA origami structure, a self-avoiding ring polymer simulation model, and nonparametric estimation statistics. The method overcomes the complexities set forth by previously used methods. We designed the DNA origami nano structures and measured the ring ssDNA polymer conformations using atomic force microscopy. We then calculated their radius of gyration, which was used as a fitting parameter for finding the persistence length. As there is no simple formulation for the radius of gyration distribution, we developed a simulation program consisting of a self-avoiding ring

SUBMITTER: Roth Weizman E 

PROVIDER: S-EPMC9247474 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature