Single transcription factor efficiently leads human induced pluripotent stem cells to functional microglia.

ABSTRACT:

Background

Microglia are innate immune cells that are the only residential macrophages in the central nervous system. They play vital physiological roles in the adult brain and during development. Microglia are particularly in the spotlight because many genetic risk factors recently identified for neurodegenerative diseases are largely expressed in microglia. Rare polymorphisms in these risk alleles lead to abnormal activity of microglia under traumatic or disease conditions.

Methods

In the present study, to investigate the multifaceted functions of human microglia, we established a novel robust protocol to generate microglia from human induced pluripotent stem cells (hiPSCs) using a combination of cytokines and small chemicals essential for microglia ontogeny. Moreover, we

SUBMITTER: Sonn I 

PROVIDER: S-EPMC9248164 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature