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ABSTRACT: Synopsis
We describe a rapid and easily deployed method for sensitive amino measurement in biological samples.
SUBMITTER: Cunningham G
PROVIDER: S-EPMC9248210 | biostudies-literature | 2022 Jun
REPOSITORIES: biostudies-literature
Cunningham G G Zhou Yun Y Summar M M
Molecular genetics and metabolism reports 20220420
We have developed a fast and accurate method that uses a small volume of sample to determine over 25 of the typically reported amino acids in human plasma. Samples were prepped with a single step using a spin filter to remove proteins, avoiding the decreased sensitivity from dilution in acid precipitation. Using a reverse phase (RP) High Performance Liquid Chromatography (HPLC) system with O-phthaldehyde (OPA) as the pre-column derivatization reagent, and UV detection at 338 nm, we did a direct ...[more]