High-resolution melting (HRM) curve analysis as a potential tool for the identification of earthworm species and haplotypes.

ABSTRACT:

Background

Earthworm communities are an important component of soil biodiversity and contribute to a number of ecosystem functions such as soil-nutrient cycling. Taxonomic identification is an essential requirement to assess earthworm biodiversity and functionality. Although morphological identification of species is labour-intensive, it is the most commonly used method due to a lack of cost-efficient alternatives. Molecular approaches to identify earthworms at species and haplotype level such as DNA barcoding are gaining popularity in science but are rarely applied in practice. In contrast to barcoding, the differentiation of PCR products based on their thermal denaturation properties using high-resolution melting (HRM) curve analysis is a fast and cost-efficient molecular closed-

SUBMITTER: Vaupel A 

PROVIDER: S-EPMC9248783 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature