Sequence-assignment validation in cryo-EM models with checkMySequence.

ABSTRACT: The availability of new artificial intelligence-based protein-structure-prediction tools has radically changed the way that cryo-EM maps are interpreted, but it has not eliminated the challenges of map interpretation faced by a microscopist. Models will continue to be locally rebuilt and refined using interactive tools. This inevitably results in occasional errors, among which register shifts remain one of the most difficult to identify and correct. Here, checkMySequence, a fast, fully automated and parameter-free method for detecting register shifts in protein models built into cryo-EM maps, is introduced. It is shown that the method can assist model building in cases where poorer map resolution hinders visual interpretation. It is also shown that checkMySequence could have helped to avoi

SUBMITTER: Chojnowski G 

PROVIDER: S-EPMC9248842 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature