PSL-LCCL: a resource for subcellular protein localization in liver cancer cell line SK_HEP1.

ABSTRACT: The characterization of subcellular protein localization provides a basis for further understanding cellular behaviors. A delineation of subcellular localization of proteins on cytosolic membrane-bound organelles in human liver cancer cell lines (hLCCLs) has yet to be performed. To obtain its proteome-wide view, we isolated and enriched six cytosolic membrane-bound organelles in one of the hLCCLs (SK_HEP1) and quantified their proteins using mass spectrometry. The vigorous selection of marker proteins and a machine-learning-based algorithm were implemented to localize proteins at cluster and neighborhood levels. We validated the performance of the proposed method by comparing the predicted subcellular protein localization with publicly available resources. The profiles enabled investigatin

SUBMITTER: Huang F 

PROVIDER: S-EPMC9248857 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature