Evolution of transcription factor binding through sequence variations and turnover of binding sites.

ABSTRACT: Variations in noncoding regulatory sequences play a central role in evolution. Interpreting such variations, however, remains difficult even in the context of defined attributes such as transcription factor (TF) binding sites. Here, we systematically link variations in cis-regulatory sequences to TF binding by profiling the allele-specific binding of 27 TFs expressed in a yeast hybrid, in which two related genomes are present within the same nucleus. TFs localize preferentially to sites containing their known consensus motifs but occupy only a small fraction of the motif-containing sites available within the genomes. Differential binding of TFs to the orthologous alleles was well explained by variations that alter motif sequence, whereas differences in chromatin accessibility betwee

SUBMITTER: Krieger G 

PROVIDER: S-EPMC9248875 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature