Native RNA sequencing in fission yeast reveals frequent alternative splicing isoforms.

ABSTRACT: The unicellular yeast Schizosaccharomyces pombe (fission yeast) retains many of the splicing features observed in humans and is thus an excellent model to study the basic mechanisms of splicing. Nearly half the genes contain introns, but the impact of alternative splicing in gene regulation and proteome diversification remains largely unexplored. Here we leverage Oxford Nanopore Technologies native RNA sequencing (dRNA), as well as ribosome profiling data, to uncover the full range of polyadenylated transcripts and translated open reading frames. We identify 332 alternative isoforms affecting the coding sequences of 262 different genes, 97 of which occur at frequencies >20%, indicating that functional alternative splicing in S. pombe is more prevalent than previously suspecte

SUBMITTER: Montanes JC 

PROVIDER: S-EPMC9248878 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature