Single-cell RNA sequencing in silent corticotroph tumors confirms impaired POMC processing and provides new insights into their invasive behavior.

ABSTRACT:

Objective

Provide insights into the defective POMC processing and invasive behavior in silent pituitary corticotroph tumors.

Design and methods

Single-cell RNAseq was used to compare the cellular makeup and transcriptome of silent and active corticotroph tumors.

Results

A series of transcripts related to hormone processing peptidases and genes involved in the structural organization of secretory vesicles were reduced in silent compared to active corticotroph tumors. Most relevant to their invasive behavior, silent corticotroph tumors exhibited several features of epithelial-to-mesenchymal transition, with increased expression of mesenchymal genes along with the loss of transcripts that regulate hormonal biogenesis and secretion. Silent corticotroph tumor vascular smoo

SUBMITTER: Zhang D 

PROVIDER: S-EPMC9248914 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature