Bothrops lanceolatus snake venom impairs mitochondrial respiration and induces DNA release in human heart preparation.

ABSTRACT:

Introduction

Envenomations by Bothrops snakebites can induce overwhelming systemic inflammation ultimately leading to multiple organ system failure and death. Release of damage-associated molecular pattern molecules (DAMPs), in particular of mitochondrial origin, has been implicated in the pathophysiology of the deregulated innate immune response.

Objective

To test whether whole Bothrops lanceolatus venom would induce mitochondrial dysfunction and DAMPs release in human heart preparations.

Methods

Human atrial trabeculae were obtained during cannulation for cardiopulmonary bypass from patients who were undergoing routine coronary artery bypass surgery. Cardiac fibers were incubated with vehicle and whole Bothrops lanceolatus venom for 24hr before high-resolution respi

SUBMITTER: Cano-Sanchez M 

PROVIDER: S-EPMC9249236 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature