Unknown

Dataset Information

0

Peptide mediated, enhanced toxicity of a bacterial pesticidal protein against southern green stink bug.

ABSTRACT: The damage caused by stink bugs that feed on agricultural crops accounts for such significant losses that transgenic plant resistance to stink bugs would be highly desirable. As the level of toxicity of the Bacillus thuringiensis-derived, ETX/Mtx2 pesticidal protein Mpp83Aa1 is insufficient for practical use against the southern green stink bug Nezara viridula, we employed two disparate approaches to isolate peptides NvBP1 and ABP5 that bind to specific proteins (alpha amylase and aminopeptidase N respectively) on the surface of the N. viridula gut. Incorporation of these peptides into Mpp83Aa1 provided artificial anchors resulting in increased gut binding, and enhanced toxicity. These peptide-modified pesticidal proteins with increased toxicity provide a key advance for potential future use against N. viridula when delivered by transgenic plants to mitigate economic loss associated with this important pest.

SUBMITTER: Banerjee R 

PROVIDER: S-EPMC9249324 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Peptide mediated, enhanced toxicity of a bacterial pesticidal protein against southern green stink bug.

Banerjee Rahul R   Flores-Escobar Biviana B   Chougule Nanasaheb P NP   Cantón Pablo Emiliano PE   Dumitru Razvan R   Bonning Bryony C BC  

Microbial biotechnology 20220322 7

The damage caused by stink bugs that feed on agricultural crops accounts for such significant losses that transgenic plant resistance to stink bugs would be highly desirable. As the level of toxicity of the Bacillus thuringiensis-derived, ETX/Mtx2 pesticidal protein Mpp83Aa1 is insufficient for practical use against the southern green stink bug Nezara viridula, we employed two disparate approaches to isolate peptides NvBP1 and ABP5 that bind to specific proteins (alpha amylase and aminopeptidase  ...[more]

Similar Datasets

Genomics Tissue of Nezara viridula, the southern green stink bug
| PRJNA762988 | ENA
Unknown RNAi-induced knockdown of white gene in the southern green stink bug (Nezara viridula L.).
| S-EPMC9213411 | biostudies-literature
Unknown Identification and comparison of key RNA interference machinery from western corn rootworm, fall armyworm, and southern green stink bug.
| S-EPMC6124762 | biostudies-literature
Unknown Role of reactive oxygen species and isoflavonoids in soybean resistance to the attack of the southern green stink bug.
| S-EPMC7502232 | biostudies-literature
Unknown Distinct properties of proteases and nucleases in the gut, salivary gland and saliva of southern green stink bug, Nezara viridula.
| S-EPMC4901325 | biostudies-literature
Unknown Comparing the Feeding Damage of the Invasive Brown Marmorated Stink Bug to a Native Stink Bug and Leaffooted Bug on California Pistachios.
| S-EPMC7599547 | biostudies-literature
Unknown Multiple concurrent and convergent stages of genome reduction in bacterial symbionts across a stink bug family.
| S-EPMC8032781 | biostudies-literature
Unknown Unveiling detoxifying symbiosis and dietary influence on the Southern green shield bug microbiota.
| S-EPMC11585277 | biostudies-literature
Unknown Cannibalism in the Brown Marmorated Stink Bug Halyomorpha halys (Stal).
| S-EPMC7563328 | biostudies-literature
Unknown Substantially altered bacterial diversity associated with developmental stages of litchi stink bug, <i>Tessaratoma javanica</i> (Thunberg) (Hemiptera: Tessaratomidae).
| S-EPMC11219338 | biostudies-literature