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Aglycone sterics-selective enzymatic glycan remodeling.


ABSTRACT: Precision remodeling of glycans in their native environments is pivotal for understanding glycan-mediated biological events and has important biotechnological implications in fields of clinical diagnosis, glyco-immune checkpoint therapy, and so forth. However, the influence of aglycone-steric diversity on the selectivity of glycan remodeling has been largely overlooked, limiting the application in complex biological scenarios. Here, we report the achievement of aglycone sterics-selective enzymatic glycan remodeling by controlled grafting of functional polymers from glycoenzyme. Through tuning polymer length, a series of enzyme-polymer composites with varying substrate permeability are prepared, which afford an activity pattern-based differentiation strategy for aglycone sterics. This leads to the implementation of glycolipid's partner screening, and aglycone sterics-selective glycan remodeling in a complex biological environment. We further orchestrate the polymer length adjustment with external cues to regulate aglycone-steric selectivity in a multi-faceted fashion, resulting in an unexpected enhancement of glycolipid remodeling, and temporal control of glycan remodeling on live cells.

SUBMITTER: Mao A 

PROVIDER: S-EPMC9249669 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature

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Aglycone sterics-selective enzymatic glycan remodeling.

Mao Anwen A   Zhang Yan Y   Wang Guyu G   Zhong Tong T   Chen Xinyu X   Wang Haiqi H   Xie Ran R   Wang Xiaojian X   Ding Lin L   Ju Huangxian H  

iScience 20220613 7


Precision remodeling of glycans in their native environments is pivotal for understanding glycan-mediated biological events and has important biotechnological implications in fields of clinical diagnosis, glyco-immune checkpoint therapy, and so forth. However, the influence of aglycone-steric diversity on the selectivity of glycan remodeling has been largely overlooked, limiting the application in complex biological scenarios. Here, we report the achievement of aglycone sterics-selective enzymat  ...[more]

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