MICU1 controls spatial membrane potential gradients and guides Ca²⁺ fluxes within mitochondrial substructures.

ABSTRACT: Mitochondrial ultrastructure represents a pinnacle of form and function, with the inner mitochondrial membrane (IMM) forming isolated pockets of cristae membrane (CM), separated from the inner-boundary membrane (IBM) by cristae junctions (CJ). Applying structured illumination and electron microscopy, a novel and fundamental function of MICU1 in mediating Ca²⁺ control over spatial membrane potential gradients (SMPGs) between CM and IMS was identified. We unveiled alterations of SMPGs by transient CJ openings when Ca²⁺ binds to MICU1 resulting in spatial cristae depolarization. This Ca²⁺/MICU1-mediated plasticity of the CJ further provides the mechanistic bedrock of the biphasic mitochondrial Ca²⁺ uptake kinetics via the mitochondrial Ca²⁺

SUBMITTER: Gottschalk B 

PROVIDER: S-EPMC9249747 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature