Protocol for three alternative paradigms to test spatial learning and memory in mice.

ABSTRACT: Here, we describe three alternative paradigms to overcome the limitations of the most widely used spatial learning paradigm for rodents: the Morris water maze. We outline the preparation of behavioral testing rooms and mouse handling/habituation prior to testing. We then detail three spatial learning and memory tasks: the Barnes maze, active place avoidance, and novel object location tasks. These tests have been successfully used across multiple ages (from 2 to 24 months) in both wild-type and transgenic animals. For complete details on the use and execution of this protocol, please refer to Leiter et al. (2022).

SUBMITTER: Blackmore DG 

PROVIDER: S-EPMC9249832 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature