Optimization of industrial (3000 L) production of Bacillus subtilis CW-S and its novel application for minituber and industrial-grade potato cultivation.

ABSTRACT: A commercial plant probiotic product was developed employing Bacillus subtilis CW-S in submerged fermentation. The effects of molasses and urea on cell growth were investigated with the goal of low-cost manufacturing. Plackett-Burman and Central-Composite Design (CCD) were utilized to optimize production parameters to maximize productivity. The stability of the formulated product and its efficacy in cultivating minituber in aeroponics and industrial-grade potatoes in the field were assessed. The results showed that the medium BS10 (molasses and urea) produced satisfactory cell density (7.19 × 10⁸ CFU/mL) as compared to the control (1.51 × 10⁷ CFU/mL) and BS1-BS9 (expensive) media (1.84 × 10⁷-1.37 × 10⁹ CFU/mL). According to validated CCD results,

SUBMITTER: Abuhena M 

PROVIDER: S-EPMC9249890 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature