Molecular docking and nucleotide sequencing of successive expressed recombinant fungal peroxidase gene in E.coli.

ABSTRACT:

Background

Fungal peroxidases are oxidoreductases that utilize hydrogen peroxide to catalyze lignin biodegradation.

Results

PER-K (peroxidase synthesis codon gene) was transformed from Aspergillus niger strain AN512 deposited in the National Center for Biotechnology Information with the accession number OK323140 to Escherichia coli strain (BL21-T7 with YEp356R recombinant plasmid) via calcium chloride heat-shock method. The impact of four parameters (CaCl₂ concentrations, centrifugation time, shaking speed, growth intensity) on the efficacy of the transformation process was evaluated. Furthermore, peroxidase production after optimization was assessed both qualitatively and quantitatively, as well as SDS-PAGE analysis. The optimum conditions for a successful transf

SUBMITTER: Khedr M 

PROVIDER: S-EPMC9249955 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature