Toxicity monitoring of solvents, hydrocarbons, and heavy metals using statistically optimized model of luminous Vibrio sp. 6HFE.

ABSTRACT:

Background

The utilization of bioluminescent bacteria in environmental monitoring of water contaminates considers being a vital and powerful approach. This study aimed to isolate, optimize, and apply luminescent bacteria for toxicity monitoring of various toxicants in wastewater.

Results

On the basis of light intensity, strain Vibrio sp. 6HFE was initially selected, physiologically/morphologically characterized, and identified using the 16SrDNA gene. The luminescence production was further optimized by employing statistical approaches (Plackett-Burman design and central composite design). The maximum bioluminescence intensity recorded 1.53 × 10⁶ CPS using optimized medium containing (g/L), yeast extract (0.2g), CaCl₂ (4.0), MgSO₄ (0.1), and K

SUBMITTER: Hassan H 

PROVIDER: S-EPMC9249957 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature