PDGFRα⁺ Interstitial Cells are Effector Cells of PACAP Signaling in Mouse and Human Colon.

ABSTRACT:

Background & aims

Platelet-derived growth factor receptor α (PDGFRα)-positive interstitial cells (PIC) are interposed between enteric nerve fibers and smooth muscle cells (SMCs) in the tunica muscularis of the gastrointestinal tract. PIC have robust expression of small conductance Ca²⁺ activated K⁺ channels 3 (SK3 channels) and transduce inhibitory inputs from purinergic and sympathetic nerves in mouse and human colon. We investigated whether PIC also express pituitary adenylate cyclase-activating polypeptide (PACAP) receptors, PAC1 (PAC₁R), and are involved in mediating inhibitory regulation of colonic contractions by PACAP in mouse and human colons.

Methods

Gene expression analysis, Ca²⁺ imaging, and contractile experiments were

SUBMITTER: Kurahashi M 

PROVIDER: S-EPMC9250024 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature