Comprehensive functional genomic analyses link APC somatic mutation and mRNA-miRNA networks to the clinical outcome of stage-III colorectal cancer patients.

ABSTRACT:

Background

Colorectal cancer (CRC) is a major health concern globally, but exhibits regional and/or environmental distinctions in terms of outcome especially for patients with stage III CRC.

Methods

From 2014 to 2016, matched pairs of tumor and adjacent normal tissue samples from 60 patients with stage I-IV CRC from Chang Gung Memorial Hospital in Taiwan were analyzed using next-generation sequencing. The DNA, mRNA, and miRNA sequences of paired tumor tissues were profiled. An observational study with survival analysis was done. Online datasets of The Cancer Genome Atlas (TCGA) and The International Cancer Genome Consortium (ICGC) were also integrated and compared.

Results

The gene that exhibited the highest mutation rate was adenomatous polyposis coli (APC) (75.0%),

SUBMITTER: Chiang SF 

PROVIDER: S-EPMC9250073 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature