Flagella disruption in Bacillus subtilis increases amylase production yield.

ABSTRACT:

Background

Bacillus subtilis is a Gram-positive bacterium used as a cell factory for protein production. Over the last decades, the continued optimization of production strains has increased yields of enzymes, such as amylases, and made commercial applications feasible. However, current yields are still significantly lower than the theoretically possible yield based on the available carbon sources. In its natural environment, B. subtilis can respond to unfavorable growth conditions by differentiating into motile cells that use flagella to swim towards available nutrients.

Results

In this study, we analyze existing transcriptome data from a B. subtilis α-amylase production strain at different time points during a 5-day fermentation. We observe that genes of the fla/che operon

SUBMITTER: Fehler AO 

PROVIDER: S-EPMC9250202 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature