Proteomic response of Turicibacter bilis MMM721 to chicken bile and its bile acids.

ABSTRACT:

Objective

Bile and its individual components, mainly bile acids, are important for digestion and drive bacterial community dynamics in the upper gastrointestinal tract of chickens. However, specific responses to bile acids have been characterized in only a few commensal bacteria, and it is unclear how other members of the microbiota respond to biliary stress. Here, we used label-free LC-MS/MS to assess the proteomic response of a common inhabitant of the chicken small intestine, Turicibacter bilis MMM721, to 24 h of growth in anaerobic growth media supplemented with 0.1% whole chicken bile, 0.1% taurochenodeoxycholic acid (TCDCA), or 0.1% taurocholic acid (TCA).

Results

Seventy, 46, and 10 differentially expressed proteins were identified in Turicibacter bilis MMM721 culture

SUBMITTER: Maki JJ 

PROVIDER: S-EPMC9250206 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature