Characterization of hydrogen sulfide toxicity to human corneal stromal fibroblasts.

ABSTRACT: Hydrogen sulfide gas (H₂ S) is a chemical weapon and a common environmental pollutant. H₂ S intoxication is lethal to humans and animals. H₂ S contact to the eye can cause vision loss. However, the molecular mechanisms associated with H₂ S toxicity to the cornea remain unclear, and no specific therapy exists to mitigate ocular damage from H₂ S. Here, we report H₂ S-induced cytotoxicity and the parameters contributing to the molecular mechanisms associated with corneal toxicity using primary human corneal stromal fibroblasts (hCSFs) in vitro. Sodium hydrosulfide (NaSH) was used as a source of H₂ S, and the cytotoxicity of H₂ S was determined by treating hCSF cells with varying concentrations of NaSH (0-1

SUBMITTER: Balne PK 

PROVIDER: S-EPMC9250278 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature