Endoplasmic reticulum-to-Golgi trafficking of procollagen III via conventional vesicular and tubular carriers.

ABSTRACT: Collagen is the major protein component of the extracellular matrix. Synthesis of procollagens starts in the endoplasmic reticulum (ER), and three α chains form a rigid triple helix 300-400 nm in length. It remains unclear how such a large cargo is transported from the ER to the Golgi apparatus. In this study, to elucidate the intracellular transport of fibril-forming collagens, we fused cysteine-free GFP to the N-telopeptide region of procollagen III (GFP-COL3A1) and analyzed transport by live-cell imaging. We found that the maturation dynamics of procollagen III was largely different from that of network-forming procollagen IV. Proline hydroxylation of procollagen III uniquely triggered the formation of intralumenal droplet-like structures, similarly to events caused by liquid-liquid pha

SUBMITTER: Hirata Y 

PROVIDER: S-EPMC9250382 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature