Retinoic acid induced meiosis initiation in female germline stem cells by remodelling three-dimensional chromatin structure.

ABSTRACT:

Objectives

This study aimed to clarify the regulation and mechanism of meiotic initiation in FGSC development.

Materials and methods

FGSCs were induced to differentiate into meiosis in differentiation medium. RNA sequencing was performed to analysis the difference of transcription level. High-through chromosome conformation capture sequencing (Hi-C) was performed to analysis changes of three-dimensional chromatin structure. Chromosome conformation capture further confirmed a spatial chromatin loop. ChIP-qPCR and dual luciferase reporter were used to test the interaction between Stimulated by retinoic acid gene 8 (STRA8) protein and Trip13 promoter.

Results

Compared with FGSCs, the average diameter of STRA8-positive germ cells increased from 13 μm to 16.8 μm. Furthermo

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC9251051 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature