A combinatorial strategy to identify various types of QTLs for quantitative traits using extreme phenotype individuals in an F₂ population.

ABSTRACT: Theoretical and applied studies demonstrate the difficulty of detecting extremely over-dominant and small-effect genes for quantitative traits via bulked segregant analysis (BSA) in an F₂ population. To address this issue, we proposed an integrated strategy for mapping various types of quantitative trait loci (QTLs) for quantitative traits via a combination of BSA and whole-genome sequencing. In this strategy, the numbers of read counts of marker alleles in two extreme pools were used to predict the numbers of read counts of marker genotypes. These observed and predicted numbers were used to construct a new statistic, G_w, for detecting quantitative trait genes (QTGs), and the method was named dQTG-seq1. This method was significantly better than existing BSA methods. I

SUBMITTER: Li P 

PROVIDER: S-EPMC9251438 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature