Covering the Combinatorial Design Space of Multiplex CRISPR/Cas Experiments in Plants.

ABSTRACT: Over the past years, CRISPR/Cas-mediated genome editing has revolutionized plant genetic studies and crop breeding. Specifically, due to its ability to simultaneously target multiple genes, the multiplex CRISPR/Cas system has emerged as a powerful technology for functional analysis of genetic pathways. As such, it holds great potential for application in plant systems to discover genetic interactions and to improve polygenic agronomic traits in crop breeding. However, optimal experimental design regarding coverage of the combinatorial design space in multiplex CRISPR/Cas screens remains largely unexplored. To contribute to well-informed experimental design of such screens in plants, we first establish a representation of the design space at different stages of a multiplex CRI

SUBMITTER: Van Huffel K 

PROVIDER: S-EPMC9251496 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature