High-resolution in situ structure determination by cryo-electron tomography and subtomogram averaging using emClarity.

ABSTRACT: Cryo-electron tomography and subtomogram averaging (STA) has developed rapidly in recent years. It provides structures of macromolecular complexes in situ and in cellular context at or below subnanometer resolution and has led to unprecedented insights into the inner working of molecular machines in their native environment, as well as their functional relevant conformations and spatial distribution within biological cells or tissues. Given the tremendous potential of cryo-electron tomography STA in in situ structural cell biology, we previously developed emClarity, a graphics processing unit-accelerated image-processing software that offers STA and classification of macromolecular complexes at high resolution. However, the workflow remains challenging, especially for newcomers to the fiel

SUBMITTER: Ni T 

PROVIDER: S-EPMC9251519 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature