Healing Ability of Central Corneal Epithelium in Rabbit Ocular Surface Injury Models.

ABSTRACT:

Purpose

Wound healing of the corneal epithelium mainly involves two types of cells: limbal stem/progenitor cells (LSCs) and differentiated central corneal epithelial cells (CECs). The healing ability of CECs is still debatable, and its correlated transcriptomic alterations during wound healing are yet to be elucidated. This study aimed to determine the healing ability and mechanisms underlying the actions of CECs using rabbit ocular surface injury models.

Methods

A central corneal ring-like residual epithelium model was used to investigate the healing ability of CECs. Uninjured and injury-stimulated LSCs and CECs were collected for transcriptomic analysis. The analysis results were verified by quantitative reverse transcriptase polymerase chain reaction, immunofluorescence s

SUBMITTER: Zhang W 

PROVIDER: S-EPMC9251814 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature