Genome-wide identification of functional enhancers and their potential roles in pig breeding.

ABSTRACT:

Background

The pig is an economically important livestock species and is a widely applied large animal model in medical research. Enhancers are critical regulatory elements that have fundamental functions in evolution, development and disease. Genome-wide quantification of functional enhancers in the pig is needed.

Results

We performed self-transcribing active regulatory region sequencing (STARR-seq) in the porcine kidney epithelial PK15 and testicular ST cell lines, and reliably identified 2576 functional enhancers. Most of these enhancers were located in repetitive sequences and were enriched within silent and lowly expressed genes. Enhancers poorly overlapped with chromatin accessibility regions and were highly enriched in chromatin with the repressive histone modificatio

SUBMITTER: Wu Y 

PROVIDER: S-EPMC9252078 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature