Production of nascent ribosome precursors within the nucleolar microenvironment of Saccharomyces cerevisiae.

ABSTRACT: 35S rRNA transcripts include a 5'-external transcribed spacer followed by rRNAs of the small and large ribosomal subunits. Their processing yields massive precursors that include dozens of assembly factor proteins. In Saccharomyces cerevisiae, nucleolar assembly factors form 2 coaxial layers/volumes around ribosomal DNA. Most of these factors are cyclically recruited from a latent state to an operative state, and are extensively conserved. The layers match, at least approximately, known subcompartments found in higher eukaryotic cells. ∼80% of assembly factors are essential. The number of copies of these assembly factors is comparable to the number of nascent transcripts. Moreover, they exhibit "isoelectric balance," with RNA-binding candidate "nucleator" assembly factors being notably bas

SUBMITTER: Lin S 

PROVIDER: S-EPMC9252279 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature