REVERSE: a user-friendly web server for analyzing next-generation sequencing data from in vitro selection/evolution experiments.

ABSTRACT: Next-generation sequencing (NGS) enables the identification of functional nucleic acid sequences from in vitro selection/evolution experiments and illuminates the evolutionary process at single-nucleotide resolution. However, analyzing the vast output from NGS can be daunting, especially with limited programming skills. We developed REVERSE (Rapid EValuation of Experimental RNA Selection/Evolution) (https://www.reverseserver.org/), a web server that implements an integrated computational pipeline through a graphical user interface, which performs both pre-processing and detailed sequence level analyses within minutes. Raw FASTQ files are quality-filtered, dereplicated, and trimmed before being analyzed by either of two pipelines. The first pipeline counts, sorts, and tracks enrichment of u

SUBMITTER: Weiss Z 

PROVIDER: S-EPMC9252737 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature