DNAzymeBuilder, a web application for in situ generation of RNA/DNA-cleaving deoxyribozymes.

ABSTRACT: Nucleic acid cleaving DNAzymes are versatile and robust catalysts that outcompete ribozymes and protein enzymes in terms of chemical stability, affordability and ease to synthesize. In spite of their attractiveness, the choice of which DNAzyme should be used to cleave a given substrate is far from obvious, and requires expert knowledge as well as in-depth literature scrutiny. DNAzymeBuilder enables fast and automatic assembly of DNAzymes for the first time, superseding the manual design of DNAzymes. DNAzymeBuilder relies on an internal database with information on RNA and DNA cleaving DNAzymes, including the reaction conditions under which they best operate, their kinetic parameters, the type of cleavage reaction that is catalyzed, the specific sequence that is recognized by the DNAzyme, t

SUBMITTER: Mohammadi-Arani R 

PROVIDER: S-EPMC9252740 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature