PatcHwork: a user-friendly pH sensitivity analysis web server for protein sequences and structures.

ABSTRACT: pH regulates protein function and interactions by altering the charge of individual residues causing loss or gain of intramolecular noncovalent bonds, which may lead to structural rearrangements. While tools to analyze residue-specific charge distribution of proteins at a given pH exist, currently no tool is available to investigate noncovalent bond changes at two different pH values. To make protein pH sensitivity analysis more accessible, we developed patcHwork, a web server that combines the identification of amino acids undergoing a charge shift with the determination of affected noncovalent bonds at two user-defined pH values. At the sequence-only level, patcHwork applies the Henderson-Hasselbalch equation to determine pH-sensitive residues. When the 3D protein structure is available,

SUBMITTER: Schmitz M 

PROVIDER: S-EPMC9252814 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature