Simiate and the focal adhesion kinase FAK1 cooperate in the regulation of dendritogenesis.

ABSTRACT: Despite the crucial importance of dendritogenesis for the correct functioning of neurons, the molecular mechanisms underlying neuronal arborisation are still not well understood. Current models suggest that distinct parts and phases of dendritic development are regulated by the expression of distinct transcription factors, that are able to target the cytoskeleton. Two proteins recently implicated in dendritogenesis are the Focal Adhesion Kinase FAK1 and the Actin-binding protein Simiate. Using heterologous expression systems as well as mouse brain extracts in combination with coprecipitation assays, we show that Simiate is able to associate with FAK1. Differential centrifugation experiments further revealed the interaction to be present in cytosolic as well as nuclear fractions. Inside the

SUBMITTER: Rama R 

PROVIDER: S-EPMC9253104 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature