Isotope-labeled amyloid-β does not transmit to the brain in a prion-like manner after peripheral administration.

ABSTRACT: Findings of early cerebral amyloid-β deposition in mice after peripheral injection of amyloid-β-containing brain extracts, and in humans following cadaveric human growth hormone treatment raised concerns that amyloid-β aggregates and possibly Alzheimer's disease may be transmissible between individuals. Yet, proof that Aβ actually reaches the brain from the peripheral injection site is lacking. Here, we use a proteomic approach combining stable isotope labeling of mammals and targeted mass spectrometry. Specifically, we generate ¹³ C-isotope-labeled brain extracts from mice expressing human amyloid-β and track ¹³ C-lysine-labeled amyloid-β after intraperitoneal administration into young amyloid precursor protein-transgenic mice. We detect injected amyloid-β in the liv

SUBMITTER: Brackhan M 

PROVIDER: S-EPMC9253763 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature