Role of the DmpR-mediated regulatory circuit in bacterial biodegradation properties in methylphenol-amended soils.

ABSTRACT: Pathway substrates and some structural analogues directly activate the regulatory protein DmpR to promote transcription of the dmp operon genes encoding the (methyl)phenol degradative pathway of Pseudomonas sp. strain CF600. While a wide range of phenols can activate DmpR, the location and nature of substituents on the basic phenolic ring can limit the level of activation and thus utilization of some compounds as assessed by growth on plates. Here we address the role of the aromatic effector response of DmpR in determining degradative properties in two soil matrices that provide different nutritional conditions. Using the wild-type system and an isogenic counterpart containing a DmpR mutant with enhanced ability to respond to para-substituted phenols, we demonstrate (i) that the enhanced i

SUBMITTER: Sarand I 

PROVIDER: S-EPMC92538 | biostudies-literature | 2001 Jan

REPOSITORIES: biostudies-literature