Proteomic profiling of serum small extracellular vesicles reveals immune signatures of children with pneumonia.

ABSTRACT:

Background

Pneumonia is the leading cause of death in young children globally. However, the underlying pathological mechanism of pediatric pneumonia remains unclear. In infection disease contexts, small extracellular vesicles (sEVs) have been shown to be a useful source of markers for pathogenesis and immune response. We hypothesized that functional molecules such as protein harbored by sEVs would provide mechanistic insights into the immune response in children with pneumonia.

Methods

We isolated sEVs from serum collected from children with and without pneumonia, performed proteomic analysis of the sEVs with label-free mass spectrometry, and then conducted functional enrichment analysis of proteomic data.

Results

We identified fifteen differentially expressed protein

SUBMITTER: Cheng J 

PROVIDER: S-EPMC9253949 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature