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A beginner's guide to assembling a draft genome and analyzing structural variants with long-read sequencing technologies.


ABSTRACT: Advances in long-read DNA sequencing technologies have enabled researchers to obtain high-quality genomes and finely resolve structural variants (SVs) in many species, even from small laboratories. The hands-on protocol presented here will guide you through the process of analyzing three different types of publicly available Drosophila melanogaster datasets obtained using current long-read sequencing technologies. We hope that this protocol will help in guiding researchers who are new to the process of long-read sequencing analysis.

SUBMITTER: Kim J 

PROVIDER: S-EPMC9254108 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature

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A beginner's guide to assembling a draft genome and analyzing structural variants with long-read sequencing technologies.

Kim Jun J   Kim Chuna C  

STAR protocols 20220629 3


Advances in long-read DNA sequencing technologies have enabled researchers to obtain high-quality genomes and finely resolve structural variants (SVs) in many species, even from small laboratories. The hands-on protocol presented here will guide you through the process of analyzing three different types of publicly available Drosophila melanogaster datasets obtained using current long-read sequencing technologies. We hope that this protocol will help in guiding researchers who are new to the pro  ...[more]

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