ToxCast chemical library Wnt screen identifies diethanolamine as an activator of neural progenitor proliferation.

ABSTRACT: Numerous autism spectrum disorder (ASD) risk genes are associated with Wnt signaling, suggesting that brain development may be especially sensitive to genetic perturbation of this pathway. Additionally, valproic acid, which modulates Wnt signaling, increases risk for ASD when taken during pregnancy. We previously found that an autism-linked gain-of-function UBE3A ^T485A mutant construct hyperactivated canonical Wnt signaling, providing a genetic means to elevate Wnt signaling above baseline levels. To identify environmental use chemicals that enhance or suppress Wnt signaling, we screened the ToxCast Phase I and II libraries in cells expressing this autism-linked UBE3A ^T485A gain-of-function mutant construct. Using structural comparisons, we

SUBMITTER: Wolter JM 

PROVIDER: S-EPMC9254222 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature