Specific (sialyl-)Lewis core 2 <i>O</i>-glycans differentiate colorectal cancer from healthy colon epithelium.

ABSTRACT: Cells are covered with a dense layer of carbohydrates, some of which are solely present on neoplastic cells. The so-called tumor-associated carbohydrate antigens (TACAs) are increasingly recognized as promising targets for immunotherapy. These carbohydrates differ from those of the surrounding non-cancerous tissues and contribute to the malignant phenotype of the cancer cells by promoting proliferation, metastasis, and immunosuppression. However, due to tumor tissue heterogeneity and technological limitations, TACAs are insufficiently explored. Methods: A workflow was established to decode the colorectal cancer (CRC)-associated O-linked glycans from approximately 20,000 cell extracts. Extracts were obtained through laser capture microdissection of formalin fixed paraffin embe

SUBMITTER: Madunic K 

PROVIDER: S-EPMC9254241 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature