Single-cell sequencing reveals CD133⁺CD44^--originating evolution and novel stemness related variants in human colorectal cancer.

ABSTRACT:

Background

Tumor heterogeneity of human colorectal cancer (CRC)-initiating cells (CRCICs) in cancer tissues often represents aggressive features of cancer progression. For high-resolution examination of CRCICs, we performed single-cell whole-exome sequencing (scWES) and bulk cell targeted exome sequencing (TES) of CRCICs to investigate stemness-specific somatic alterations or clonal evolution.

Methods

Single cells of three subpopulations of CRCICs (CD133⁺CD44⁺, CD133^-CD44⁺, and CD133⁺CD44^- cells), CRC cells (CRCCs), and control cells from one CRC tissue were sorted for scWES. Then, we set up a mutation panel from scWES data and TES was used to validate mutation distribution and clonal evolution in additional 9

SUBMITTER: Zhang X 

PROVIDER: S-EPMC9254347 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature