Hypoxia-inducible CircPFKFB4 Promotes Breast Cancer Progression by Facilitating the CRL4^DDB2 E3 Ubiquitin Ligase-mediated p27 Degradation.

ABSTRACT: Hypoxic microenvironment and circular RNAs (circRNAs) have shown critical implications in breast cancer (BC) progression. However, the specific functions and underlying mechanisms of circRNAs in BC under hypoxia remain largely unknown. We first screened for differentially expressed circRNAs in normoxic and hypoxic MCF-7 cells using circRNA microarray. A novel hypoxia-induced circRNA, circPFKFB4, was identified. Clinical investigation showed that circPFKFB4 was highly expressed in BC tissues and cell lines, and its overexpression was positively correlated with the advanced clinical stage and poor prognosis of BC patients. Functionally, circPFKFB4 promoted the proliferation of BC cells both in vitro and in vivo. Mechanistically, HIF1α bound to hypoxia response elements in the p

SUBMITTER: Chen H 

PROVIDER: S-EPMC9254479 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature