Larva migrans in BALB/c mice experimentally infected with Toxocara cati ensured by PCR assay.

ABSTRACT:

Background

Toxocara cati, the cat roundworm, is a parasitic nematode that known to cause toxocariasis in intermediate hosts and humans. In this study, we characterized the dynamics of T. cati larvae migration in BALB/c mice after inoculation with eggs and ensured the migration detecting the larval DNA by a PCR. To evaluate the dynamics of larval migration and distribution, twenty-four BALB/c mice were orally inoculated with 2500 T. cati infective eggs and the visceral organs of the infected animals were examined by pepsin digestion and microscopic parasite counts, followed by PCR at day 1 to 28 post-inoculation.

Results

The PCR assays were successfully used for detection of T. cati larvae in tissue samples and T. cati larvae and the DNAs were found in the liver, lungs, heart

SUBMITTER: Naderbandi M 

PROVIDER: S-EPMC9254581 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature