Nanoscale architecture and coordination of actin cores within the sealing zone of human osteoclasts.

ABSTRACT: Osteoclasts are unique in their capacity to degrade bone tissue. To achieve this process, osteoclasts form a specific structure called the sealing zone, which creates a close contact with bone and confines the release of protons and hydrolases for bone degradation. The sealing zone is composed of actin structures called podosomes nested in a dense actin network. The organization of these actin structures inside the sealing zone at the nano scale is still unknown. Here, we combine cutting-edge microscopy methods to reveal the nanoscale architecture and dynamics of the sealing zone formed by human osteoclasts on bone surface. Random illumination microscopy allowed the identification and live imaging of densely packed actin cores within the sealing zone. A cross-correlation analysis of the fl

SUBMITTER: Portes M 

PROVIDER: S-EPMC9255968 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature