Identification of Candidate Genes Responsible for Flower Colour Intensity in Gentiana triflora.

ABSTRACT: Gentians cultivated in Japan (Gentiana triflora and Gentiana scabra and hybrids) have blue flowers, but flower colour intensity differs among cultivars. The molecular mechanism underlying the variation in flower colour intensity is unclear. Here, we produced F₂ progeny derived from an F₁ cross of intense- and faint-blue lines and attempted to identify the genes responsible for flower colour intensity using RNA-sequencing analyses. Comparative analysis of flower colour intensity and transcriptome data revealed differentially expressed genes (DEGs), although known flavonoid biosynthesis-related genes showed similar expression patterns. From quantitative RT-PCR (qRT-PCR) analysis, we identified two and four genes with significantly different expression leve

SUBMITTER: Tasaki K 

PROVIDER: S-EPMC9257217 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature