VaAPL1 Promotes Starch Synthesis to Constantly Contribute to Soluble Sugar Accumulation, Improving Low Temperature Tolerance in Arabidopsis and Tomato.

ABSTRACT: ADP-glucose pyrophosphorylase (AGPase) is a key rate-limiting enzyme involved in starch synthesis. APL1, an AGPase large subunit, plays an important role in the growth and development of grapes; however, its function in withstanding low temperature (LT) remains elusive. Hence, VaAPL1 was cloned from Vitis amurensis (Zuoshan I), and its function was characterized. The gene was highly expressed in the phloem of V. amurensis during winter dormancy (0, -5, and - 10°C). Phylogenetic relationships demonstrated that VaAPL1 was closely genetic related to SlAPL1 (from Solanum lycopersicum), and clustered into I group. Further, VaAPL1 was ectopically expressed in Arabidopsis thaliana (ecotype Columbia, Col) and tomato ("Micro-Tom" tomato) to characterize its

SUBMITTER: Liang G 

PROVIDER: S-EPMC9257282 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature