EHMT2 (G9a) activation in mantle cell lymphoma and its associated DNA methylation and gene expression.

ABSTRACT:

Objective

The function of euchromatic histone-lysine N-methyltransferase 2 (EHMT2) has been studied in several cancers; however, little is known about its role in mantle cell lymphoma (MCL). Thus, this study aimed to characterize the significance and function of EHMT2 in MCL.

Methods

EHMT2 expression in MCL and reactive hyperplasia (RH) were investigated by immunohistochemistry. Genome-wide analysis of DNA methylation was performed on EHMT2 + MCL samples. The function of EHMT2 was determined by CCK8, flow cytometry, and western blot assays. Gene expression profile analysis was performed before and after EHMT2 knockdown to search for EHMT2-regulated genes. Co-immunoprecipitation (Co-IP) experiments were conducted to identify the proteins interacting with EHMT2.

Resu

SUBMITTER: Wang J 

PROVIDER: S-EPMC9257318 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature