[Therapeutic mechanism of emodin for treatment of rheumatoid arthritis: a network pharmacology-based analysis].

ABSTRACT:

Objective

To investigate the therapeutic mechanism of emodin in the treatment of rheumatoid arthritis (RA) using a network pharmacology-based method and validate this mechanism in a fibroblast-like synovial cell line.

Methods

The PubChem, Targetnet, SwissTargetPrediction, Genecards, OMIM, and DisGeNET databases were searched to obtain emodin targets and RA-related genes. A protein-protein interaction (PPI) network was constructed, and GO and KEGG pathway enrichment analyses were carried out to analyze the intersection genes. AutoDock4.2.6 software was used to simulate molecular docking between emodin and its candidate targets. In a cultured fibroblast-like synovial cell line (MH7A), the effects of different concentrations of emodin on proliferation of tumor necrosis factor-<

SUBMITTER: Cao C 

PROVIDER: S-EPMC9257365 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature