Sequence and thermodynamic characteristics of terminators revealed by FlowSeq and the discrimination of terminators strength.

ABSTRACT: The intrinsic terminator in prokaryotic forms secondary RNA structure and terminates the transcription. However, leaking transcription is common due to varied terminator strength. Besides of the representative hairpin and U-tract structure, detailed sequence and thermodynamic features of terminators were not completely clear, and the effect of terminator on the upstream gene expression was unclearly. Thus, it is still challenging to use terminator to control expression with higher precision. Here, in E. Coli, we firstly determined the effect of the 3'-end sequences including spacer sequences and terminator sequences on the expression of upstream and downstream genes. Secondly, terminator mutation library was constructed, and the thermodynamic and sequence features differing in the t

SUBMITTER: Zhai W 

PROVIDER: S-EPMC9257418 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature