RT-qPCR as a screening platform for mutational and small molecule impacts on structural stability of RNA tertiary structures.

ABSTRACT: The exponential increase in the discovery and characterization of RNA tertiary structures has highlighted their active role in a variety of human diseases, yet often their interactome and specific function remain unknown. Small molecules offer opportunities to both decode these cellular roles and develop therapeutics, however there are few examples of small molecules that target biologically relevant RNA tertiary structures. While RNA triple helices are a particularly attractive target, discovery of triple helix modulators has been hindered by the lack of correlation between small molecule affinity and effect on structural modulation, thereby limiting the utility of affinity-based screening as a primary filtering method. To address this challenge, we developed a high-throughput RT-qPCR scr

SUBMITTER: Zafferani M 

PROVIDER: S-EPMC9257624 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature